Canada's Denis Shapovalov, right, was Wednesday's biggest casualty after losing to American Frances Tiafoe ©Getty Images

Home players Denis Shapovalov and Felix Auger-Aliassime are both out of the men's Canadian Open, following second-round upsets in Toronto.

The line-ups for the third round of the men’s and women’s singles tournaments at the Canadian Open are now complete - but no Canadians remain in the singles at the Association of Tennis Professionals (ATP) Tour Masters 1000.

Fifth seed Shapovalov fell 6-1, 6-4 to the United States' Frances Tiafoe and Auger-Aliassime was eliminated 7-5, 6-4 by Serbia's Dušan Lajović.

Four further seeded players were eliminated in their first matches of the tournament.

Alex de Minaur of Australia was trounced 6-1, 6-1 by Georgia’s Nikoloz Basilashvili, Chile’s Cristian Garín lost to John Isner of the US despite taking the first set, Olympic silver medallist Karen Khachanov beat  15th seed and fellow Russian Aslan Karatsev in two sets, while Australia’s James Duckworth is the last remaining qualifier after upsetting Italy’s Jannik Sinner.

Duckworth will now face top seed Daniil Medvedev of Russia after the world number two progressed yesterday.

Kei Nishikori of Japan withdrew from the tournament with a shoulder injury to hand Poland’s Hubert Hurkacz a walkover as he seeks his second ATP Tour Masters 1000 win of the year, while fourth seed Andrey Rublev from Russia is safely through after beating Italy’s Fabio Fognini in straight sets.

In the Women’s Tennis Association 1000 event in Montreal, top seed Aryna Sabalenka of Belarus recovered from losing her serve early in the deciding third set to beat 2018 Canadian Open finalist Sloane Stephens7-6 (7-4), 4-6, 6-4.

Ukraine's Elina Svitolina won bronze at Tokyo 2020, but is one of three top-six seeds to have been eliminated in their first match at the Canadian Open ©Getty Images
Ukraine's Elina Svitolina won bronze at Tokyo 2020, but is one of three top-six seeds to have been eliminated in their first match at the Canadian Open ©Getty Images

Two of the top six seeds and former winners of Canadian Open could not avoid an early elimination, however - this after fifth seed Garbiñe Muguruza of Spain was knocked out yesterday.

Olympic bronze medallist and 2017 champion when the women’s event was held in Toronto, Elina Svitolina, took the first set against Britain’s Johanna Konta, but the three-time major semi-finalist hit back to clinch the final two sets and progress.

Romania’s Simona Halep, winner the last two times the women’s singles event has been held in Montreal in 2016 and 2018, was also defeated despite taking the first set, losing to American Danielle Collins.

Czech fourth seed Karolína Plíšková in contrast came from behind to beat Croatia’s Donna Vekić in a deciding-set tiebreak, and the hosts will have two representatives in the round of 16 after wildcard entry Rebecca Marino came from a set down to beat Spain’s Paula Badosa 1-6, 7-5, 6-4.

Marino has set up a clash with Sabalenka, and joins defending champion and second seed Bianca Andreescu who progressed yesterday in representing Canada.

Andreescu will face Tunisia’s Ons Jabeur as she recovered from losing the first set to see off Russia’s Daria Kasatkina.