Roman Berbr has resigned as deputy head of the Czech Football Association (FAČR) after he was one of 20 people detained in connection with a match-fixing investigation.
Berbr sent his resignation via his attorney 24 hours after he was charged with match-fixing following raids conducted across the the country by criminal investigators.
According to reports in the Czech media, the officials detained are accused of influencing matches in the second and third tiers of football in the nation through referees.
The scandal has also prompted the FAČR to dismiss its entire Referees Commission, including chairman Jozef Chovanec.
Prague chief prosecutor Lenka Bradacova said the criminal case had been opened "on the activities of a criminal group related to corruption and influence on the results of football matches".
FAČR chairman Martin Malik confirmed UEFA was aware of the developments, which have plunged Czech football into crisis.
"I have said several times that I am terrified of what would happen if Roman Berbr’s end in football came suddenly and unexpectedly," Malik told local media.
"I just hope that football can recover from this change in a positive sense.
"All of us, who are active in Czech football, we let this happen.
"The situation which we are dealing with now is abnormal.
"It is an attack against the very essence of football.
"I cannot tell how big the impact will be."
The FAČR has ordered a review of the finances of regional chapters, with a special focus on the area Berbr represented, as part of a seven-point plan to address the scandal.
"We need to put in place measures to prevent similar situations," Malik added.
"If Czech football is to win, we must come together, talk constructively together and come up with solutions."