Paralympic champions Michael Gallagher, Susan Powell and David Nicholas head-up a 14-member squad selected by Cycling Australia for the Para Track World Championships in Montichiari, Italy, in March.
Thirty-seven-year-old Gallagher has twice stood on top of a Paralympic podium, winning a gold medal at London 2012 in the C5 individual pursuit and also triumphing at Beijing 2008 in the LC1 individual pursuit.
The Scottish-born rider also has two Paralympic bronze medals in his collection, as well as nine gold medals at World Championships on the track and one on the road.
Powell, meanwhile, is still competing at the age of 48 and won C4 individual pursuit gold at London 2012, as well as a silver in the road time trial.
The 24-year-old Nicholas, who claimed glory at London 2012 in the C3 road time trial, also took home a bronze medal in the C1-3 road race.
The trio will be joined in the Australia team by dual road and track world champion Alistair Donohoe and Paralympic medallists Alex Linsey and Simone Kennedy.
Amanda Reid, Darcy Thompson, Hannah Macdougall and Kyle Bridgwood will all make their debuts, while three men's tandem teams have been named, including 2014 world champions Matt Formston and pilot Mick Curran.
Seven-time Paralympian Kieran Modra and pilot David Edwards, and Commonwealth Games medallist Paul Kennedy and Pilot Nicholas Yallouris, will also compete.
Jessica Gallagher, who competed at the London Paralympics, has also been selected to ride with pilot Madison Janssen.
The 29-year-old has won two bronze medals at Winter Paralympics, coming third in the visually impaired giant slalom at both Vancouver 2010 and Sochi 2014.
The Championships in Montichiari are due to take place between March 17 and 20, and will be a key build-up event to this year's Paralympics in Rio de Janeiro.